Software:Article Title: Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.
Article Snippet: .. Growth Medium was Reagents and Tools table (continued) Reagent/Resource Reference or Source Identifier or Catalog Number Software R https://r-project.org 3.3.3 Knime https://www.knime.com 3.2.1 Python https://python.org 3.4 Zeiss Zen blue http://zeiss.com 2012 Zeiss Zen black http://zeiss.com 2012 LabView http://ni.com 2013 SP1 Fiji/ImageJ https://fiji.sc fiji-latest, timestamp 20160902160412 MATLAB https://www.mathworks.com/ 2015a IPA https://www.qiagenbioinformatics.com/produc ts/ingenuity-pathway-analysis/ Other Axio Observer.Z1 Carl Zeiss Microscopy 431007 9901 000 Scan Module LSM 710 Base Unit Carl Zeiss Microscopy 000000 1410 052 Spectral Detection Unit LSM 780 + 2 PMT Carl Zeiss Microscopy 000000 1670 619 Objective Plan-Apochromat 20×/0.8 M27 Carl Zeiss Microscopy 420650 9901 000 Laser diode 405 nm CW 30 mW Carl Zeiss Microscopy 000000 1410 119 Laser 561 nm Carl Zeiss Microscopy 000000 1410 117 Master Beam Splitter Wheel for Laser 633, 561, 514, 488, 458 nm Carl Zeiss Microscopy 000000 1583 982 Beam path NLO+405/445 Axio Observer/Axio Imager Carl Zeiss Microscopy 000000 1741 731 Spinning Disc Unit CSU-X1A 5000 Carl Zeiss Microscopy 423638 9090 000 Spinning Disc Laser Unit 405 nm Carl Zeiss Microscopy 000000 1514 464 Rolera EM-C2 Bio-Imaging Microscopy Camera, Mono, 14-bit Carl Zeiss Microscopy 000000 1915 668 PROcellcare 5030 + Peristaltic Pump Extension Unit PROdesign n/a Energenie EG-PM2 Energenie EG-PM2 4× LabTek holder EMBLEM LTT-01 and LTT-02 a 2018 The Authors Molecular Systems Biology 14: e8238 | 2018 9 of 15 D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2024 from IP 2401:4900:1ce0:1da:bd52:d1ad:dafb:bc03. adapted from Debnath et al (2003) and is based on DMEM/F12 (no phenol red, Gibco 21041-33), with 5% Horse Serum (Gibco 16050-122), 20 ng/ml EGF (Sigma E9644-.2MG), 0.5 mg/ml Hydrocortisone (Sigma H0888-1g), 100 ng/ml Cholera Toxin (Sigma C8052-1MG), and 10 lg/ml Insulin (Life Technologies 12585014). ..
Indirect Immunoperoxidase Assay:Article Title: Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.
Article Snippet: .. Growth Medium was Reagents and Tools table (continued) Reagent/Resource Reference or Source Identifier or Catalog Number Software R https://r-project.org 3.3.3 Knime https://www.knime.com 3.2.1 Python https://python.org 3.4 Zeiss Zen blue http://zeiss.com 2012 Zeiss Zen black http://zeiss.com 2012 LabView http://ni.com 2013 SP1 Fiji/ImageJ https://fiji.sc fiji-latest, timestamp 20160902160412 MATLAB https://www.mathworks.com/ 2015a IPA https://www.qiagenbioinformatics.com/produc ts/ingenuity-pathway-analysis/ Other Axio Observer.Z1 Carl Zeiss Microscopy 431007 9901 000 Scan Module LSM 710 Base Unit Carl Zeiss Microscopy 000000 1410 052 Spectral Detection Unit LSM 780 + 2 PMT Carl Zeiss Microscopy 000000 1670 619 Objective Plan-Apochromat 20×/0.8 M27 Carl Zeiss Microscopy 420650 9901 000 Laser diode 405 nm CW 30 mW Carl Zeiss Microscopy 000000 1410 119 Laser 561 nm Carl Zeiss Microscopy 000000 1410 117 Master Beam Splitter Wheel for Laser 633, 561, 514, 488, 458 nm Carl Zeiss Microscopy 000000 1583 982 Beam path NLO+405/445 Axio Observer/Axio Imager Carl Zeiss Microscopy 000000 1741 731 Spinning Disc Unit CSU-X1A 5000 Carl Zeiss Microscopy 423638 9090 000 Spinning Disc Laser Unit 405 nm Carl Zeiss Microscopy 000000 1514 464 Rolera EM-C2 Bio-Imaging Microscopy Camera, Mono, 14-bit Carl Zeiss Microscopy 000000 1915 668 PROcellcare 5030 + Peristaltic Pump Extension Unit PROdesign n/a Energenie EG-PM2 Energenie EG-PM2 4× LabTek holder EMBLEM LTT-01 and LTT-02 a 2018 The Authors Molecular Systems Biology 14: e8238 | 2018 9 of 15 D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2024 from IP 2401:4900:1ce0:1da:bd52:d1ad:dafb:bc03. adapted from Debnath et al (2003) and is based on DMEM/F12 (no phenol red, Gibco 21041-33), with 5% Horse Serum (Gibco 16050-122), 20 ng/ml EGF (Sigma E9644-.2MG), 0.5 mg/ml Hydrocortisone (Sigma H0888-1g), 100 ng/ml Cholera Toxin (Sigma C8052-1MG), and 10 lg/ml Insulin (Life Technologies 12585014). ..
Microscopy:Article Title: Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.
Article Snippet: .. Growth Medium was Reagents and Tools table (continued) Reagent/Resource Reference or Source Identifier or Catalog Number Software R https://r-project.org 3.3.3 Knime https://www.knime.com 3.2.1 Python https://python.org 3.4 Zeiss Zen blue http://zeiss.com 2012 Zeiss Zen black http://zeiss.com 2012 LabView http://ni.com 2013 SP1 Fiji/ImageJ https://fiji.sc fiji-latest, timestamp 20160902160412 MATLAB https://www.mathworks.com/ 2015a IPA https://www.qiagenbioinformatics.com/produc ts/ingenuity-pathway-analysis/ Other Axio Observer.Z1 Carl Zeiss Microscopy 431007 9901 000 Scan Module LSM 710 Base Unit Carl Zeiss Microscopy 000000 1410 052 Spectral Detection Unit LSM 780 + 2 PMT Carl Zeiss Microscopy 000000 1670 619 Objective Plan-Apochromat 20×/0.8 M27 Carl Zeiss Microscopy 420650 9901 000 Laser diode 405 nm CW 30 mW Carl Zeiss Microscopy 000000 1410 119 Laser 561 nm Carl Zeiss Microscopy 000000 1410 117 Master Beam Splitter Wheel for Laser 633, 561, 514, 488, 458 nm Carl Zeiss Microscopy 000000 1583 982 Beam path NLO+405/445 Axio Observer/Axio Imager Carl Zeiss Microscopy 000000 1741 731 Spinning Disc Unit CSU-X1A 5000 Carl Zeiss Microscopy 423638 9090 000 Spinning Disc Laser Unit 405 nm Carl Zeiss Microscopy 000000 1514 464 Rolera EM-C2 Bio-Imaging Microscopy Camera, Mono, 14-bit Carl Zeiss Microscopy 000000 1915 668 PROcellcare 5030 + Peristaltic Pump Extension Unit PROdesign n/a Energenie EG-PM2 Energenie EG-PM2 4× LabTek holder EMBLEM LTT-01 and LTT-02 a 2018 The Authors Molecular Systems Biology 14: e8238 | 2018 9 of 15 D ow nloaded from https://w w w .em bopress.org on Septem ber 17, 2024 from IP 2401:4900:1ce0:1da:bd52:d1ad:dafb:bc03. adapted from Debnath et al (2003) and is based on DMEM/F12 (no phenol red, Gibco 21041-33), with 5% Horse Serum (Gibco 16050-122), 20 ng/ml EGF (Sigma E9644-.2MG), 0.5 mg/ml Hydrocortisone (Sigma H0888-1g), 100 ng/ml Cholera Toxin (Sigma C8052-1MG), and 10 lg/ml Insulin (Life Technologies 12585014). ..
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